Meet Our Staff

Prof. Li-Fen, Huang

Director of International Bachelor Porgram in Engineering



Ph.D. in Plant Molecular and Cellular Biology, University of Florida.

Professional Experience

  • Yuan Ze University       Chairman, International Program in Engineering for Bachelor
  • Yuan Ze University       Associate professor
  • Yuan Ze University       Assistant professor                       
  • Academia Sinica          Postdoctoral fellow                      
  • University of Florida     Postdoctoral Research Scientist  

Research Area

  • 1. Engineering protein expression system in rice cells for valuable medical protein production 
  • 2. DNA detection biosensor for GMO
  • 3. Develop plant tissue culture systems for snow lotus


1. Nguyen, T.M., Lu, C.A., Huang, L.F.* (2022) Applications of CRISPR/Cas9 in a rice protein expression system via an intron-targeted insertion approach. Plant Science, 315:111132. (SCI, Impact Factor: 4.729).

2. Wu, C.L., Lin, L.F., Hsu, H.C., Huang, L.F., Hsiao, C.D., Chou, M.L. (2021) Saussurea involucrata (Snow Lotus) ICE1 and ICE2 orthologues involved in regulating cold stress tolerance in transgenic Arabidopsis. Int. J. Mol. Sci., 22, 10850. (SCI, Impact Factor: 5.924).

3. Sinaga, D.S., Ho, S.L, Lu. C.A., Yu, S.M., Huang, L.F.* (2021) Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells. Plant Methods, 25(17):99. doi: 10.1186/s13007-021-00799-2 (SCI, Impact Factor: 4.993). 

4. Huang, L.F.*, Sinaga, D.S., Tan, C.C., Hsieh, S.J.M., Huang, C.H. (2021) Expression of recombinant human octamer-binding transcription factor 4 in rice suspension cells. Int. J. Mol. Sci., 22: 1409. (SCI, Impact Factor: 5.924).

5. Chuang, J.N., Diao, P.Y., Huang, W.S., Huang, L.F., Senapati, S., Chang, H.C. and Sun, Y.M.* (2020) Novel homogeneous anion exchange membranes for reproducible and sensitive nucleic acid detection via current–voltage characteristic measurement, ACS Applied Materials and Interfaces, 49: 54459–54472 (SCI, Impact Factor: 9.229).

6. Huang, L.F.*, Liu, Y.K., Su, S.-C., Lai, C.-C., Wu, C.-R., Chao, T.-J. Yang, Y.H. (2020) Genetic engineering of transitory starch accumulation by knockdown of OsSEX4 in rice plants for enhanced bioethanol production, Biotechnol Bioeng, 117: 933-944 (SCI, Impact Factor: 4.53).

7. Liu, Y.K., Lu, C.W., Chang, J.Y., Lu, C.F., Tan, C.C. and Huang, L.F.* (2018) Optimization of the culture medium for recombinant protein production under the control of the αAmy3 promoter in a rice suspension-cultured cell expression system, Plant Cell, Tissue and Organ Culture, 132: 383–391. (SCI, Impact Factor: 2.711).

8. Huang, L.F., Lin, K.H., He, S.L., Chen, J.L., Jiang, J.Z., Chen, B.H., Hou, Y.S., Chen, R.S., Hong, C.Y. and Ho, S.H.* (2016) Multiple patterns of regulation and overexpression of a ribonuclease-like pathogenesis-related protein gene, OsPR10a, conferring disease resistance in rice and Arabidopsis. PLoS ONE, 11(6): e0156414. doi:10.1371/journal.pone.0156414. (SCI, Impact Factor: 3.240).

9. Huang, C.K., Shen, Y.L., Huang, L.F., Wu, S.J., Yeh, C.H., Lu, C.A.* (2016) The DEAD-Box RNA Helicase AtRH7/PRH75 Participates in Pre-rRNA Processing, Plant Development and Cold Tolerance in Arabidopsis. Plant Cell Physiol., 57(1):174-91. doi: 10.1093/pcp/pcv188. (SCI, Impact Factor: 4.927).

10. Huang, L.F.*, Tan, C.C., Yeh, J.F., Liu, H.Y., Liu, Y.K., Ho, S.L., Lu, C.A. (2015) Efficient secretion of recombinant proteins from rice suspension cultured cells modulated by the choice of signal Peptide. PLoS ONE, 10(10): e0140812. doi:10.1371/journal.pone.0140812 (SCI, Impact Factor: 3.240).

11. Huang, L.F.*; Lin, J.Y.; Pan, K.Y.; Huang, C.K.; Chu, Y.K. (2015) Overexpressing Ferredoxins in Chlamydomonas reinhardtii increase starch and oil yields and enhance electric power production in a photo microbial fuel cell. Int. J. Mol. Sci., 16: 19308-19325, Link: (SCI, Impact Factor: 5.924).

12. Huang, C.K., Lo, P.C., Huang, L.F., Wu, S.J., Yeh, C.H., Lu, C.A.* (2015) A single-repeat MYB transcription repressor, MYBH, participates in regulation of leaf senescence in Arabidopsis. Plant Mol Biol, 88(3):269-86. doi: 10.1007/s11103-015-0321-2. (SCI, Impact Factor: 4.076).

13. Liu, Y.K., Li, Y.T., Lu, C.F., Huang, L.F.* (2015) Enhancement of recombinant human serum albumin in transgenic rice cell culture system by cultivation strategy. New Biotechnology, 32(3): 328-334 (SCI, Impact Factor: 5.079).

14. Lin. Y.H., Huang. L.F., Hase, T., Huang, H. E., Feng, T.Y. (2015) Expression of plant ferredoxin-like protein (PFLP) enhances tolerance to heat stress in Arabidopsis thaliana. New Biotechnolgy, 32(2): 235-242 (SCI, Impact Factor: 5.079).

15. Chou, W.L., Huang, L.F., Fang, J.C., Yeh, C.H., Hong, C.Y., Wu, S.J., Lu, C.A. (2014) Divergence of the expression and subcellular localization of CCR4‑associated factor 1 (CAF1) deadenylase proteins in Oryza sativa. Plant Mol Biol, 85(4-5): 443-458 (SCI, Impact Factor: 4.076).

16. Chen, W.C., Lai, Y.A., Lin, Y.C., Ma, J.W., Huang, L.F., Yang, N.S., Ho, C.T., Kuo, S.C., Way, T.D. (2013) Curcumin suppresses Doxorubicin-induced epithelial-mesenchymal transition via the inhibition of TGF-β and PI3K/AKT signaling pathways in triple-negative breast cancer cells. Journal of Agricultural and Food Chemistry, 61(48): 11817~11824 (SCI, Impact factor 5.279).

17. Chen. C.C., Chen, Y.; Hsi, Y.T.; Chang, C.S.; Huang, L.F., Ho, C.T., Way T.D., Kao, J.Y. (2013) Chemical constituents and anticancer activity of Curcuma zedoaria Roscoe essential oil against non-small cell lung carcinoma cells in vitro and in vivo. Journal of Agricultural and Food Chemistry, 61(47): 11418~11427 (SCI, Impact factor 5.279).

18. Lin, Y.H., Pan, K.Y., Hung, C.H., Huang, H.E., Chen, C.L., Feng, T.Y., Huang, L.F.* (2013) Over-expression of Ferredoxin, PETF, enhances tolerance to heat stress in Chlamydomonas reinhardtii. Int. J. Mol. Sci.,14(10) 20913-20929. Link: (SCI, Impact factor: 5.924).

19. Kuo, Y.C., Tan, C.C., Ku, J.T., Hsu, W.C., Su, S.C., Lu, C.A., Huang, L.F.* (2013) Improving Pharmaceutical Protein Production in Oryza sativa. Int. J. Mol. Sci., 14: 8719-8739. Link: (SCI, Impact factor: 5.924).

20. Ho, S.L., Huang, L.F., Lu, C.A., Wang, C.C., Chen, J and Yu, S.M. (2013) Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings. Plant Mol Biol, 81:347-361 (equal contribution as the first author, SCI, Impact Factor: 4.076).

21. Liu, Y.K., Huang, L.F., Ho, S.L., Liao, C.Y., Liu, H.Y., Lai, Y.H., Yu, S.M. and Lu, C.A. (2012) Production of mouse granulocyte-macrophage colony stimulating factor by Gateway technology and transgenic rice cell culture. Biotechnology & Bioengineering, 109:1239-1247 (SCI, Impact Factor: 4.53).

22. Lo, Y.C., Huang, L.F., Cheng, C.L., Chen, J. and Chang, J.S. (2011) Using a starch-rich mutant of Arabidopsis thaliana as feedstock for fermentative hydrogen production. Bioresource Technology, 102:8543-8546. (SCI, Impact factor: 9.642).

23. Huang, C.K., Huang, L.F., Huang, J.J., Wu, S.J., Yeh, C.H., Lu, C.A. (2010) A DEAD-box protein, AtRH36, is essential for female gametophyte development and is involved in rRNA biogenesis in Arabidopsis. Plant and Cell Physiology, 51:694-706 (SCI, Impact factor: 4.927).

24. Lin, H.H., Huang, L.F., Su, H.C., Jeng. S.T. (2009) Effects of the multiple polyadenylation signal AAUAAA on mRNA 3'-end formation and gene expression. Planta, 230:699-712 (equal contribution as the first author, SCI, Impact Factor: 4.116).

25. Lu, C.A., Lin, C.C., Lee, K.W., Chen, J.L., Huang, L.F., Ho, S.L., Liu, H.J., Hsing, Y.I. and Yu, S.M. (2007) The SnRK1A protein kinase plays a key role in sugar signaling during germination and seedling growth of Rice. Plant Cell, 19: 2484-2499 (SCI, Impact Factor: 11.277).

26. Huang, L.F., Bocock, P.N., Davis , J.M. and Koch, K.E. (2007) Invertase regulation: a “suite” of transcriptional and post-transcriptional mechanisms. Functional Plant Biology, 34:499-507 (SCI, Impact Factor: 3.101).

27. Kohorn, B.D., Kobayashi, M., Johansen, S., Riese, J., Huang, L.F., Koch, K., Fu, S., Dotson, A. and Byers, N. (2006) An Arabidopsis cell wall-associated kinase required for invertase activity and cell growth. Plant Journal, 46:307-316 (SCI, Impact Factor: 6.486).

28. Huang, L.F., Liu, Y.K., Lu, C.A., Hsieh, S.L. and Yu, S.M. (2005) Production of human serum albumin by sugar starvation induced promoter and rice cell culture. Transgenic Research, 14:569-581 (SCI, Impact Factor: 2.788).

29. Chiang, C.M., Yeh, F.S., Huang, L.F., Tseng, T.H., Chung M.C., Wang C.S., Lur, H.S., Shaw, J.F. and Yu, S.M. (2005) Expression of a bi-functional and thermostable amylopullulanase in transgenic rice seeds leads to autohydrolysis and altered composition of starch. Molecular Breeding, 15:125-143 (SCI, Impact Factor: 2.589).

30. Chen, M.H., Huang, L.F., Li, H.M., Chen, Y.R, Yu, S.M. (2004) Signal peptide-dependent targeting of a rice α-amylase and cargo proteins to plastids and extracellular compartments of plant cells. Plant Physiology, 135: 1367-1377 (equal contribution as the first author, SCI, Impact Factor: 8.34).

* indicates corresponding author

Note: Information of Impact Factor is from Journal Citation Reports™ 2020

-Funding from National Science Council and Ministry of Science and Technology (2008~2022)

-Young Scholar Award of Yuan Ze University (2016)

-Funding from National Science Council (2008~2020)

-Travel Grant Award from graduate student council of University of Florida, 2006,    Gainesville, Florida, U.S.A. (May 2006)

-Graduate School Travel Grant Award in Plant Biology 2005, American Society of Plant Biologists, Seattle, Washington, U.S.A. (July 2005)

-Best-Presentation Award at state-wide workshop for Plant Molecular & Cellular Biology, Daytona, Florida, U.S.A. (May 2005)



2. ROC patent, No. 530503, [修飾的啟動子序列及其應用],專利權人:元智大學;發明人:黃麗芬、陳鵬文、余淑美